htb  (ATCC)

Journal: iScience

Article Title: Synthetic zipper mediated pre-targeting system for near-infrared photoimmunotherapy

doi: 10.1016/j.isci.2025.114558

Figure Lengend Snippet: The pre-targeting complex of NIR-PIT induces the release of ICD markers (A) Flow cytometric histograms show cell surface expression of calreticulin, HSP70, and HSP90 compared to untreated controls in SKOV3 cells with scFv-tisotumab-Zip2-Zip1-SNAP-IR700. (B) OVCAR3 cells treated with scFv-Sacituzumab-Zip2-Zip1-SNAP-IR700. (C) IGROV1 cells treated with scFv-Farletuzumab-Zip2-Zip1-SNAP-IR700. Only viable cells were included in the analysis. Bar graphs show the mean fluorescence intensity (MFI) of cell surface calreticulin, HSP70, and HSP90. Data are represented as mean ± SD from three biological replicates. Cells exposed to NIR light irradiation without incubation with NIR-PIT agents were used as control. Statistical significance was determined by a one-way ANOVA and Dunnett's test. ∗∗∗ p ≤ 0.001, ∗∗∗∗ p ≤ 0.0001.

Article Snippet: Ovarian cancer cell lines SKOV3 (HTB-77), OVCAR3 (HTB-161), IGROV1 (SCC-203), A2780 (ECACC-93112519), and HEK293T (CRL-11268) were obtained from the American Type Culture Collection, the European Collection of Authenticated Cell Cultures, and Sigma-Aldrich.

Techniques: Expressing, Fluorescence, Irradiation, Incubation, Control

Journal: iScience

Article Title: Synthetic zipper mediated pre-targeting system for near-infrared photoimmunotherapy

doi: 10.1016/j.isci.2025.114558

Figure Lengend Snippet: The pre-targeting complex of NIR-PIT induces the release of ICD markers (A) Flow cytometric histograms show cell surface expression of calreticulin, HSP70, and HSP90 compared to untreated controls in SKOV3 cells with scFv-tisotumab-Zip2-Zip1-SNAP-IR700. (B) OVCAR3 cells treated with scFv-Sacituzumab-Zip2-Zip1-SNAP-IR700. (C) IGROV1 cells treated with scFv-Farletuzumab-Zip2-Zip1-SNAP-IR700. Only viable cells were included in the analysis. Bar graphs show the mean fluorescence intensity (MFI) of cell surface calreticulin, HSP70, and HSP90. Data are represented as mean ± SD from three biological replicates. Cells exposed to NIR light irradiation without incubation with NIR-PIT agents were used as control. Statistical significance was determined by a one-way ANOVA and Dunnett's test. ∗∗∗ p ≤ 0.001, ∗∗∗∗ p ≤ 0.0001.

Article Snippet: SKOV3 , ATCC , HTB-77.

Techniques: Expressing, Fluorescence, Irradiation, Incubation, Control

Journal: Cell Death & Disease

Article Title: Transglutaminase 2 exacerbates ovarian cancer survival by directly inactivating GSK3β

doi: 10.1038/s41419-026-08447-0

Figure Lengend Snippet: A Conventional chemotherapies increase TGase 2 levels and decrease GSK3β levels. OVCAR‑3 or SKOV‑3 cells were treated with cisplatin (DDP, 1 mM) or paclitaxel (PTX, 10 μM) for 24 h, after which TGase 2 and GSK3β protein levels were measured by immunoblotting. B Chemotherapy treatment enhances the migratory ability of ovarian cancer cells. Cell migration was quantified after treatment with DDP or PTX in OVCAR-3 and SKOV-3 cells. The graphs show mean ± standard deviation (SD). Assays used three samples per group ( n = 3). C To assess metastasis suppression and the anti-tumor effects of first-line ovarian cancer therapy combined with a TGase 2 inhibitor, we performed tests using an orthotopic model using OVCAR‑8/Luc cells. Pharmacological inhibition of TGase 2 works synergistically with conventional chemotherapies and prolongs survival in an ovarian cancer mouse model. Mice ( n ≥ 8 per group) received phosphate-buffered saline (PBS), streptonigrin (STN; 0.01 mg/kg), DDP (1 mg/kg), PTX (1 mg/kg), STN + DDP, or STN + PTX. Kaplan–Meier survival curves are shown; median survival was calculated using Kaplan–Meier statistics. D Quantification of histological scores for GSK3β in ovarian cancer patient tissue using inForm™ image analysis software. Tumors were classified as stage I ( n = 83), stage II ( n = 24), stage III–IV ( n = 37), or metastatic lesions ( n = 36). Representative immunohistochemical (IHC) images are included (Supplementary Fig. ) scale bar, 100 µm. E Comparison of TG2 and GSK3β expression patterns during tumor development and metastasis. The TMA analysis graphs are presented as mean ± standard error of the mean (SEM). Multiple comparisons among three or more groups were performed with one-way analysis of variance (ANOVA). Statistical significance was set at ** p < 0.01, *** p < 0.001, and **** p < 0.0001. ns, not significant.

Article Snippet: Ovarian Cancer cell lines, including OVCAR3 (HTB-161), SKOV-3 (HTB-77), and HEK-293 (CRL-1573) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Western Blot, Migration, Standard Deviation, Inhibition, Saline, Software, Immunohistochemical staining, Comparison, Expressing

Journal: bioRxiv

Article Title: Keratin 5 marks cancer-propagating cells sustained by an osteopontin-producing niche in high-grade serous ovarian carcinoma

doi: 10.64898/2026.01.28.702332

Figure Lengend Snippet: A and B , Organoids derived from SKOV3 cells expressing both lentiviruses (A, GFP and mCherry, orange) or GFP alone (B, green). Scale bar, 100 µm. C , Quantification of KRT5+ (blue symbols, pink bars) and KRT5- (pink symbols, yellow bars) cancer organoids in 6 consecutive passages. All error bars denote s.d. D, Volume of tumors formed by serially diluted (1 x 10 5 , 1 x 10 4 , 1 x 10 3 ) of KRT5+ and KRT5- cells after their s.c. transplantation into different flanks of NSG mice. KRT5-group did not form tumors. E and F , mCherry (E) and KRT5 (F) expression in KRT5+ cell derived xenografts. Elite ABC method. Hematoxylin counterstaining. Scale bar, 60 µm. All error bars denote s.d. G. Live microscopy of cells were isolated by FACS based on their expression of GFP (green) and mCherry (magenta) after coinfection with Lenti-UbC-GFP and Lenti-KRT5mCherry. Orange, Overlay. Individual frames of live microscopy. Scale bar, 60 µm.

Article Snippet: Human ovarian cancer cell lines SKOV3 (ATCC, HTB-77), CAOV3 (ATCC, HTB-75), and CAOV4 (ATCC, HTB-76) were obtained from the American Type Culture Collection.

Techniques: Derivative Assay, Expressing, Transplantation Assay, Microscopy, Isolation

Journal: bioRxiv

Article Title: Keratin 5 marks cancer-propagating cells sustained by an osteopontin-producing niche in high-grade serous ovarian carcinoma

doi: 10.64898/2026.01.28.702332

Figure Lengend Snippet: A and B. Quantification of cells expressing KRT5 and/or SPP1 cells within human HGSC cases from single-cell RNA sequencing. Significance by Mann-Whitney U test. C. RT-PCR analysis of SPP1 expression in KRT5+ and KRT5- subpopulations of SKOV3 cells. D and E, KRT5 (green) and OPN (red) expression in HGSC (D) and primary HGSC organoid (E). Double immunofluorescence, counterstaining with DAPI (blue). Scale bar, (D) 60 µm, E (40 µm). F and G , OPN treatment increases frequency (F) and size (G) of HGSC organoids (n=3). H - K . Effect of cisplatin on frequency (H and J) and size (I and K) of organoids either transduced with SPP1 shRNA (H and I) or treated with OPN (I and K). All organoids were measured 72 hours after treating with cisplatin at different concentrations. All error bars denote s.d.

Article Snippet: Human ovarian cancer cell lines SKOV3 (ATCC, HTB-77), CAOV3 (ATCC, HTB-75), and CAOV4 (ATCC, HTB-76) were obtained from the American Type Culture Collection.

Techniques: Expressing, RNA Sequencing, MANN-WHITNEY, Reverse Transcription Polymerase Chain Reaction, Immunofluorescence, Transduction, shRNA